Localization of salivary amylase and epithelial membrane antigen in salivary gland tumours by means of immunoperoxidase and immunogold-silver techniques.

A series of 87 salivary glands was examined using an immunogold-silver technique for salivary gland amylase (SGA) and epithelial membrane antigen (EMA) and an indirect immunoperoxidase technique for both antigens. The specimens comprised 21 pleomorphic adenomas, 3 monomorphic adenomas, 25 adenolymphomas, 5 mucoepidermoid carcinomas, 3 acinic cell tumours, 8 adenoid cystic carcinomas, 2 adenocarcinomas and 8 undifferentiated carcinomas. In addition 5 normal salivary glands and 6 salivary glands showing benign lymphoepithelial lesions were included.

Ectopic production of beta-human chorionic gonadotropin by inverted papilloma of the nose.

A series of 19 inverted papillomas (transitional type or Schneiderian papillomas) of the nose were examined using an immunogold-silver (IGS) technique and an indirect immunoperoxidase technique. We demonstrated beta-HCG production in all 19 specimens with both methods. The staining demonstrated by the IGS technique was of a greater intensity and in a larger number of cells than that observed by the indirect immunoperoxidase method.

Immunogold-silver technique applied to showing malignant B cell infiltration of gastrointestinal tract in patients with chronic lymphocytic leukaemia and non-Hodgkin's lymphoma.

Gastric, duodenal, and rectal biopsy specimens from 20 patients with chronic lymphocytic leukaemia (CLL) and non-Hodgkin's lymphoma (NHL), not primarily of gastrointestinal origin, were examined using the immunogold-silver staining (IGSS) technique. In paraffin sections the presence of kappa and lambda surface immunoglobulin in lymphoid infiltrates was shown. Using this technique, nine patients were shown to have infiltration of the gastrointestinal mucosa by monoclonal B cells at one or more sites.

Nucleolar organizer regions in lymphomas.

Using a silver staining technique, nucleolar organizer region-associated proteins (Ag-NORs) have been studied in paraffin sections of 90 non-Hodgkin's lymphomas, five palatine tonsils and five 'reactive' lymph nodes. The method was readily applicable to these preparations and the Ag-NORs were enumerated with ease. A significant difference was found between the numbers of Ag-NORs in the nuclei of low-grade lymphomas (from a mean of 1 to 1.

An immunohistochemical and clinicopathological study of granulocytic sarcoma ('chloroma').

A small series of granulocytic sarcomas (GS) or 'chloromas' has been studied by means of conventional histology and immunohistochemistry. The latter was found to be most useful in the diagnosis and characterization of these neoplasms, which are rare in Great Britain. Polytypic antisera to leukocyte elastase and cathepsin G have proved to be most useful, giving intense staining in all cases.