The effect of colloid osmotic pressure in human spermatozoa exposed to hypoosmotic conditions.

The use of a protein source such as serum and albumin had been extensively employed as supplements of culture media for handling and culture of gametes and embryos. Protein molecules behave as colloids in solution and contribute to the osmotic pressure of fluids. The interaction of proteins in solution and spermatozoa needs to be assessed in order to determine their possible role in osmoregulation.

Assessment of two devices for in vitro preparation of human sperm.

The aim of the study was to assess and compare the efficiency of the ZSC-II versus the Sperm Select techniques in preparing human sperm from normozoospermic patients for use in intrauterine insemination and other assisted reproductive technologies. Twenty-five patients were included in the study. Semen was collected at intercourse and processed via 2 sperm preparation methods.

Viability of human semen specimens cryostored and transported at 5 C using the Bio-Tranz shipping system.

Objective: Transport of unprocessed human semen specimens from the production site to distant laboratories for andrological evaluation and clinical use requires the development of proper protocols and devices for the shipment and maintenance of sperm viability during transport. Factors such as maintenance of proper temperature and the specific diluent used are considered to affect the viability of semen specimens during transport. The Bio-Tranz shipper (ZDL, Inc.

Outcome of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) using human sperm prepared via a new standardized swim-up technique fit for office use.

Objective: Recent developments in the assisted reproductive technology (ART) areas necessitate the use of new and more efficient and acceptable modes of gamete in vitro manipulation techniques. As established via the use of various forms of ART, the natural process of fertilization has been largely bypassed and the gametes are obtained and manipulated outside of the normal means of conception. For the male, improvements in sperm quality have involved the use of various techniques that aim to recover a small percentage of healthy spermatozoa that can be further used for the different ART procedures.