Association between Gene Polymorphism and High Dose Methotrexate Toxicity in Childhood Acute Lymphoblastic Leukaemia and Non Hodgkin Malignant Lymphoma: Introducing a Haplotype based Approach.

Background: We investigated the clinical relevance of genetic variability for high dose methotrexate (HD-MTX) related toxicities in children and adolescents with acute lymphoblastic leukaemia (ALL) and non Hodgkin malignant lymphoma (NHML).

Patients And Methods: Eighty-eight children and adolescents with ALL/NHML were investigated for the influence of single nucleotide polymorphisms (SNPs) and haplotypes on HD-MTX induced toxicities.

Results: Patients with rs2838958 TT genotype had higher probability for mucositis development as compared to carriers of at least one rs2838958 C allele (OR 0.

[Prolonged survival of skin allografts in rats by injection of serum or a serum fraction extracted from pregnant rats].

This work report the partial purification of an alpha 2-macroglobulin present in the serum of allopregnant rats. Ultrogel filtration followed by immune absorption on different antisera were used giving a single band on polyacrylamide gel electrophoresis. This fraction was able to inhibit in vitro T cell cytotoxic response to allogenic cells.