Molecular typing of Cryptococcus neoformans: taxonomic and epidemiological aspects.

Pulsed-field gel electrophoresis (PFGE), randomly amplified polymorphic DNA (RAPD) analysis, serotype, and killer toxin sensitivity patterns of a wide range of saprobic, clinical, and veterinary isolates of both varieties of Cryptococcus neoformans were examined. C. neoformans var.

Further studies on the in vitro antifungal activity of amorolfine.

Minimal inhibitory concentrations (MICs) of amorolfine for 52 pathogenic yeasts or yeast-like organisms, filamentous and dimorphic fungi represented by 955 isolates were determined in agar dilution tests. Amorolfine has a broad antifungal spectrum, but unfortunately this drug is so far only available for topical use.

Cryptococcus neoformans var. gattii can be separated from var. neoformans by its ability to assimilate D-tryptophan.

An additional test to differentiate the varieties of Cryptococcus neoformans is described. It is based on the assimilation of D-tryptophan by the variety gattii.

[Tests for an early detection of pulmonary cryptococcosis by sputum culture].

Cryptococcosis is a serious opportunistic infection occurring in acquired immunodeficiency syndrome (AIDS) patients. As the number of infected patients with the human immunodeficiency virus (HIV) in Central Africa and especially in Rwanda increases, the prevalence of cryptococcosis can also be expected to rise. An earlier diagnosis and treatment will improve the prognosis of cryptococcosis.