Publications by authors named "P Moolhuijzen"

Motivation: Whole genome alignment of eukaryote species remains an important method for the determination of sequence and structural variations and can also be used to ascertain the representative non-redundant core-genome sequence of a population. Many whole genome alignment tools were first developed for the more mature analysis of prokaryote species with few current tools containing the functionality to process larger genomes of eukaryotes as well as genomes of more divergent species. In addition, the functionality of these tools becomes computationally prohibitive due to the significant compute resources needed to handle larger genomes.

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Article Synopsis
  • The study focuses on a destructive pathogen of wheat known as tan spot, caused by an ascomycete fungus, which has a highly variable genome influenced by the gain and loss of effector genes.
  • Researchers analyzed allelic variations in a specific chlorosis-encoding gene across 422 isolates from different regions and pathotypes, constructing a haplotype network to understand its evolutionary relationships.
  • Key findings include discovering a retrotransposon that disrupts gene function, identifying numerous mutations, and recognizing ToxB-like proteins in various other species, implying historical horizontal gene transfer during the evolution of these genes.
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Gene expression at the RBgh2 locus indicates involvement in cAMP/G-protein-coupled signalling and innate immunity in barley powdery mildew adult plant resistance. Barley powdery mildew is a globally significant disease, responsible for reduced grain yield and quality. A major effect adult plant resistance gene, RBgh2, was previously found in a landrace from Azerbaijan.

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Pathogen attacks elicit dynamic and widespread molecular responses in plants. While our understanding of plant responses has advanced considerably, little is known of the molecular responses in the asymptomatic 'green' regions adjoining lesions. Here, we explore gene expression data and high-resolution elemental imaging to report the spatiotemporal changes in the asymptomatic green region of susceptible and moderately resistant wheat cultivars infected with a necrotrophic fungal pathogen, Pyrenophora tritici-repentis.

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ToxA is one of the most studied proteinaceous necrotrophic effectors produced by plant pathogens. It has been identified in four pathogens (, , [formerly f. sp.

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