Publications by authors named "P Missineo"
Background: Although thousands of different chemicals have been identified in cigarette smoke, the characterization of their urinary metabolites still requires significant research. The aim of this work was to perform an untargeted metabolomic approach to a pilot cross-sectional study conducted on subjects with different smoking habits and to compare the results with those of the targeted measurement of mercapturic acids.
Methods: Urine samples from 67 adults, including 38 non-smokers, 7 electronic cigarette users, and 22 traditional tobacco smokers were collected.
Background: While tobacco smoke contains thousands of chemicals, some of which are carcinogenic to humans, the content of electronic cigarette smoke is less known. This work aimed to assess and compare the exposure associated with different smoking habits by profiling urinary mercapturic acids as biomarkers of toxic compounds.
Methods: In this pilot study, sixty-seven healthy adults with different smoking habits were investigated: 38 non-smokers (NS), 7 electronic cigarette users (ECU), and 22 traditional tobacco smokers (TTS).
Article Synopsis
- The study focuses on developing a method to measure bisphenol A (BPA) and metabolites of phthalates in human urine, as these are harmful endocrine-disrupting chemicals (EDCs).
- A high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC/ESI-MS/MS) technique was validated, showing high precision and accuracy for the measurements, although some validations for terephthalates were incomplete.
- This method can be valuable for epidemiological studies to assess exposure levels of these chemicals within the general population.
Article Synopsis
- The study investigates phototoxic reactions in patients with Erythropoietic Protoporphyria (EPP), focusing on factors like oxidative status, complement system activation, and mast cell response.
- In summer, EPP patients showed significantly higher levels of complement components C3 and factor-B compared to controls, indicating a stronger immune response.
- The findings suggest that phototoxic reactions can have systemic effects beyond the skin, highlighting the importance of understanding these responses for better clinical management and treatment options.
An LC-MS/MS method to profile urinary mercapturic acids, metabolites of electrophilic intermediates of occupational and environmental toxicants.
J Chromatogr B Analyt Technol Biomed Life Sci
June 2019
Introduction: Mercapturic acids are urinary metabolites of occupational and environmental toxicants. The aim of this work was to develop and validate an analytical assay for the determination of several urinary mercapturic acids to be used as biomarkers of exposure.
Method: An isotope dilution tandem mass spectrometric method, coupled with reversed-phase liquid chromatography, was developed for the analysis of mercapturic acids derived from several compounds, including those of benzene, toluene, 1,3-butadiene, styrene, acrylonitrile, 4-chloronitrobenzene, acrylamide, acrolein, propylene oxide, N,N-dimethylformamide, crotonaldehyde, ethylene oxide, and methylating and ethylating agents.