Publications by authors named "P May-Marquardt"

A large insert genomic library from eastern white pine (Pinus strobus) was probed for the microsatellite motifs (AC)n and (AG)n, all 10 trinucleotide motifs, and 22 of the 33 possible tetranucleotide motifs. For comparison with a species from a different subgenus, a loblolly pine (Pinus taeda) genomic library was also probed with the same set of di- and tri-nucleotide repeats and 11 of the tetranucleotide repeats. The four most abundant microsatellite motifs in both species were (AC)n, (AG)n, (AAT)n, and (ATC)n, which as a group accounted for over half the microsatellite sites investigated.

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Article Synopsis
  • An enrichment cloning method was used to isolate microsatellite loci from eastern white pine, achieving significant enrichment for (AC)n repeats compared to (ACAG)n repeats.
  • From 77 primer pairs, 19 microsatellite loci were successfully identified, with genotyping revealing an average of 5.4 alleles and 0.515 observed heterozygosity across 16 unrelated pines.
  • The study confirmed that (AC)n loci are highly variable and highlighted the effective development of SSR markers for use in genome mapping and population genetics research.
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