Tryptophan hydroxylase can be present in mast cells and nerve fibers of the rat dura mater but only mast cells contain serotonin.

Tryptophan hydroxylase-immunopositive (TPH-I) but not serotonin-I nerve fibers were observed in the rat dura mater. This tissue also contained numerous serotonin and TPH-I mast cells. The TPH appeared to be located in granules and/or enclosed in a juxta-nuclear organite.

Spontaneous and 5HT-induced cyclic contractions in superficial temporal arteries from chronic and episodic cluster headache patients.

Superficial temporal arteries (STAs) are abnormally dilated in the painful side during cluster headache (CH) attacks. We have assessed the possible dysfunction of these arteries by comparing in vitro the reactivity of STAs removed from the painful side of CH patients during a cluster of attacks with that of STAs from patients free of CH. The responses to KCl and norepinephrine (NE) of both types of arteries were similar.

Substance P, calcitonin gene-related peptide, and capsaicin release serotonin from cerebrovascular mast cells.

Rabbit leptomeningeal arteries contain granular cells resembling mast cells that frequently contact autonomic and sensory nerve profiles. In the present in vitro study, we determined whether these cells could be stimulated by substance P (SP) and calcitonin gene-related peptide (CGRP), which are stored and released by sensory C fibers. Immunohistochemistry of the middle cerebral artery showed that 5-HT was stored only in mast cell-like granules.

Immunohistochemical evidence for the absence of central neuron projection to pial blood vessels and dura mater.

The present work shows that, in rat pial vessels and dura mater, all the nerve fibres observable by confocal fluorescence microscopy belong to the peripheral nervous system. It has been postulated that central nervous structures such as the raphe nuclei and the locus coeruleus could send direct projections to meningeal blood vessels. Mature neurons, whose perikaryons and axons are entirely located within the central nervous system, express the low molecular mass neurofilament protein and not the 57,000 mol.