Evaluation of treatment with human chorionic gonadotropin at transfer of in vitro-produced beef embryos on reproductive outcomes in lactating multiparous Jersey cows after a synchronized ovulation.

Our objective was to evaluate the effect of treatment with human chorionic gonadotropin (hCG) at the time of transfer of in vitro-produced (IVP) beef embryos on pregnancy outcomes in lactating multiparous Jersey cows. Grade 1, stage 7 (expanded blastocyst), IVP beef embryos were produced from black Angus-based dams using 3 proven high-fertility Angus sires and were frozen for direct transfer. In a preliminary experiment, lactating multiparous Jersey cows were randomized to a 2 × 2 factorial arrangement of treatments to test the main effect of recipient synchronization protocol (Double-Ovsynch; DO; n = 169 vs.

Discovery, Optimization, and Biological Evaluation of Arylpyridones as Cbl-b Inhibitors.

Casitas B-lymphoma proto-oncogene-b (Cbl-b), a member of the Cbl family of RING finger E3 ubiquitin ligases, has been demonstrated to play a central role in regulating effector T-cell function. Multiple studies using gene-targeting approaches have provided direct evidence that Cbl-b negatively regulates T, B, and NK cell activation via a ubiquitin-mediated protein modulation. Thus, inhibition of Cbl-b ligase activity can lead to immune activation and has therapeutic potential in immuno-oncology.

Association of transition cow health with pregnancy per artificial insemination and pregnancy loss in Holstein cows submitted to a Double-Ovsynch protocol for first service.

This observational study was conducted to evaluate the effect of transition cow health on pregnancy per artificial insemination (P/AI) and pregnancy loss (PL) in cows submitted to a Double-Ovsynch protocol (DO) for first service. Lactating Holstein cows (n = 15,041) from one commercial dairy farm in northern Germany between January 2015 to December 2021 were enrolled into a modified Double-Ovsynch protocol (GnRH, 7 d later PGF, 3 d later GnRH, 7 d later GnRH, 7 d later PGF, 24 h later PGF, 32 h later GnRH, and 16 to 18 h later timed artificial insemination) for first service at 72 ± 3 d in milk. Pregnancy was diagnosed at 32 and 60 d post-AI via transrectal ultrasonography.

Effect of postpartum body condition score change on the pregnancy outcomes of lactating Jersey cows inseminated at first service with sexed Jersey or conventional beef semen after a synchronized estrus versus a synchronized ovulation.

Our objective was to compare insemination rate and pregnancies per artificial insemination (P/AI) of lactating Jersey cows inseminated at first service with sexed Jersey or conventional beef semen after submission to a Double-Ovsynch protocol for timed artificial insemination (TAI) versus a protocol to synchronize estrus at similar days in milk (DIM). Secondary objectives were to determine the effect of protocol synchrony and postpartum body condition score (BCS) change on P/AI. Lactating Jersey cows (n = 1,272) were allocated by odd versus even ear tag number, which was randomly allocated within the herd, within parity and semen type for submission to a Double-Ovsynch protocol (DO; n = 707) or a protocol to synchronize estrus (ED; n = 565).

The association between insemination eligibility and reproductive performance of nulliparous heifers on subsequent body weight and milk production of primiparous Holstein cows.

The objective of this retrospective cohort study was to determine the association between insemination eligibility and reproductive performance of nulliparous heifers with subsequent body weight (BW) at 30 d in milk (DIM) and milk production at wk 4, 8, and 12 of lactation of primiparous cows. The final data set included 1,849 primiparous Holstein cows from a commercial dairy herd. Data extracted from a commercial dairy herd management software program included parent average predicted transmitting abilities (PTA), bovine respiratory disease (BRD) incidence, pregnancies per artificial insemination (P/AI) at first insemination as nulliparous heifers, BW at 30 DIM, percent mature body weight (%MBW), and mean weekly milk production at wk 4, 8, and 12 of lactation.