Successful re-evaluation of broth medium T for growth of Francisella tularensis ssp. and other highly pathogenic bacteria.

Pavlovich's medium T was compared with other broadly used media and extensively checked by growth of various subspecies of Francisellatularensis as well as other risk group 3 bacteria. The medium was successfully re-evaluated as an optimal liquid medium suitable for enrichment of fastidious and/or highly pathogenic bacteria.

Decontamination of a BSL3 laboratory by hydrogen peroxide fumigation using three different surrogates for Bacillus anthracis spores.

Aims: Two independent trials investigated the decontamination of a BSL3 laboratory using vaporous hydrogen peroxide and compared the effect on spores of Bacillus cereus, Bacillus subtilis and Bacillus thuringiensis as surrogates for Bacillus anthracis spores, while spores of Geobacillus stearothermophilus served as control.

Methods And Results: Carriers containing 1·0 × 10(6) spores were placed at various locations within the laboratory before fumigation with hydrogen peroxide following a previously validated protocol. Afterwards, carriers were monitored by plating out samples on agar and observing enrichment in nutrient medium for up to 14 days.