[Lack of participation of cytoplasmic choline in the synthesis of acetylcholine in the striatal synaptosomes of the rat].

Synaptosomes prepared from Rat striatum were loaded with (14C) choline at 0 degrees C in the course of a 60 min. preincubation. After the removal, by washing, of the extracellular molecules, synaptosomes were incubated 5 min.

[Arguments in favor of the extramitochondrial origin of the acetyl radical of acetylcholine synthetized in rat striatum synaptosomes].

Synaptosomes prepared from Rat striatum were loaded with (2-14C) pyruvate at 0 degree C, in the course of a 1 hr. preincubation. When temperature was raised to 37 degrees C, the intra-synaptosomal (2-14C) pyruvate gave rise to 14CO2 but not to (14C) ACh.