Structural basis of directionality control in large serine integrases.

Large serine integrases (LSIs) catalyze unidirectional site-specific DNA recombination reactions, yet those reactions are reversed by the presence of a cognate recombination directionality factor (RDF). Mechanistic understanding of directionality control has been hampered by a lack of structural information. Here, we use cryo-electron microscopy (cryo-EM) to determine the structures of six SPbeta integrase-DNA complexes along the integrative (-RDF) and excisive (+RDF) reaction pathways, at 4.

First Clinical Feasibility and Safety Study of a Novel Multimodality Fallopian Tube Imaging Endoscope.

Study Objective: We present the results of the first feasibility and safety study of a novel multi-modality falloposcope, in 19 volunteers. The falloposcope incorporated multispectral fluorescence imaging (MFI) and optical coherence tomography (OCT) for evaluation of the fallopian tubes (FT).

Methods: Nineteen females undergoing elective salpingectomy were recruited in this IRB-approved study.

A domed window chamber for multi-modality optical imaging.

Current dorsal skin flap window chambers with flat glass windows are compatible with optical coherence tomography (OCT) and multiphoton microscopy (MPM) imaging. However, light sheet fluorescence microscopy (LSFM) performs best with a cylindrical or spherical sample located between its two 90° objectives and when all sample materials have the same index of refraction (). A modified window chamber with a domed viewing window made from fluorinated ethylene propylene (FEP), with n similar to water and tissue, was designed.

Multiphoton Microscopy Assessment of Healing From Tendon Laceration and Microthermal Coagula in a Rat Model.

Objectives: To study the healing response of rat Achilles tendon when lacerated or treated with intense therapeutic ultrasound (ITU) via utilization of multiphoton microscopy (MPM) imaging and histology.

Materials And Methods: The right Achilles tendon of each Sprague Dawley rat within a cohort was partially lacerated. 1 to 2 days post-surgery, each rat received ITU treatment of the Achilles tendon on either the right or left leg.

Psoas Sarcopaenia Outcomes in Elderly Patients After Acute Lower Gastrointestinal Bleeding.

Introduction Sarcopaenia has been examined as a predictor of frailty in surgical patients and may predict mortality. The hypothesis of this study is that sarcopaenia is associated with an increased risk of death following an episode of acute lower gastrointestinal bleeding (ALGB). Methods This study included patients admitted with ALGB between January 2017 and June 2022, who underwent cross-sectional imaging (CSI).