SIV monoclonal antibody administration spanning treatment interruption in macaques delays viral rebound and selects escape variants.

HIV-1 envelope broadly neutralizing antibodies represent a promising component of HIV-1 cure strategies. To evaluate the therapeutic efficacy of combination monoclonal antibodies (mAbs) in a rigorous nonhuman primate model, we tested different combinations of simian immunodeficiency virus (SIV) neutralizing mAbs in SIVmac251-infected rhesus macaques. Antiretroviral therapy-suppressed animals received anti-SIV mAbs targeting multiple Env epitopes spanning analytical treatment interruption (ATI) in 3 groups (n = 7 each): i) no mAb; ii) 4-mAb combination; and iii) 2-mAb combination.

Effectiveness of text message reminder and motivational interview on adherence to methadone treatment in Vietnam: a randomized controlled trial.

Background: Methadone maintenance treatment (MMT) is effective for treating opioid dependence. However, nonadherence can increase the risk of withdrawal syndrome, relapse, and overdose.

Methods: A community-based randomized controlled trial was conducted on 450 opioid-dependent patients undergoing MMT at three clinics in Ho Chi Minh City, Vietnam.

Semantic Memory Space Becomes Denser with Age.

Semantic memory, a repository for concepts and factual information, plays a vital role in acquiring and retrieving knowledge. This study explores the impact of age-related knowledge accumulation on semantic cognition, investigating whether a denser representational space affects retrieval processes. Using a semantic feature verification task, we employ both behavioral (reaction time; RT) and neurophysiological (event-related potential; ERP) measures to explore these dynamics across young and older adults.

Methods for detecting, building, and improving tryptophan mannosylation in glycoprotein structures.

Tryptophan mannosylation, the covalent addition of an α-ᴅ-mannose sugar to a tryptophan side chain, is a post-translational modification (PTM) that can affect protein stability, folding, and interactions. Compared to other forms of protein glycosylation, it is relatively uncommon but is affected by conformational anomalies and modeling errors similar to those seen in N- and O-glycans in the Protein Data Bank (PDB). In this work, we report methods for detecting, building, and improving mannose structures linked to tryptophans.

Investigating the metabolic load of monoclonal antibody production conveyed to an inducible CHO cell line using a transfer-rate online monitoring system.

Shake flasks are a foundational tool in early process development by allowing high throughput exploration of the design space. However, lack of online data at this scale can hamper rapid decision making. Oxygen transfer rate (OTR) monitoring has been readily applied as an online process characterization tool at the benchtop bioreactor scale.