Publications by authors named "P L Majano"
Background: Recently, we have identified a dysregulated protein signature in the esophageal epithelium of eosinophilic esophagitis (EoE) patients including proteins associated with inflammation and epithelial barrier function; however, the effect of proton pump inhibitor (PPI) treatment on this signature is unknown. Herein, we used a proteomic approach to investigate: (1) whether PPI treatment alters the esophageal epithelium protein profile observed in EoE patients and (2) whether the protein signature at baseline predicts PPI response.
Methods: We evaluated the protein signature of esophageal biopsies using a cohort of adult EoE (n = 25) patients and healthy controls (C) (n = 10).
Objectives: The aim of the study was to characterize the circulating immunome of patients with EoE before and after proton pump inhibitor (PPI) treatment in order to identify potential non-invasive biomarkers of treatment response.
Methods: PBMCs from 19 healthy controls and 24 EoE patients were studied using a 39-plex spectral cytometry panel. The plasmacytoid dendritic cell (pDC) population was differentially characterized by spectral cytometry analysis and immunofluorescence assays in esophageal biopsies from 7 healthy controls and 13 EoE patients.
Article Synopsis
- Proton pump inhibitors (PPIs) are the main treatment for eosinophilic esophagitis (EoE), but about 50% of patients don’t achieve histological remission after treatment.
- This study aimed to discover genetic markers that could predict how well PPIs work for EoE patients and to examine their link to disease characteristics.
- Results showed that patients treated with omeprazole had a significantly better reduction in eosinophil counts compared to other PPIs, and specific genetic variations (rs12368672 and rs167769) may influence both baseline eosinophil counts and the response to treatment.
Article Synopsis
- * Research involving ICAM-1 knockout cells reveals that it regulates the polarity of epithelial cells independently of leukocyte adhesion by interacting with an actomyosin network.
- * The study highlights the importance of the protein EBP50, which works alongside ICAM-1 to influence the organization of bile canalicular structures, suggesting new therapeutic approaches for maintaining epithelial function under inflammation.
Endoluminal functional lumen impedance planimetry (EndoFLIP) has become the gold standard to evaluate esophageal distensibility, although the study itself and its analysis present challenges. We propose here a new method to assess lower esophageal distension capacity that overcomes several limitations of prior approaches, including incomplete and corrupted EndoFLIP recordings. Esophageal distension capacity was evaluated with a 16-channel EndoFLIP in 10 controls and 14 patients with eosinophilic esophagitis (EoE).
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