Capability-based planning as an approach to defense planning is an almost infinitely complex engineered system with countless nodes and layers of interdependency, influenced by state and non-state diplomatic activities, information, military and economic actions creating secondary and third order effects. The main output of capability-based planning is the set of capability requirements needed to achieve the expected end-state. One revitalized qualitative technique that allows us to gain insights into unstructured and fuzzy problems in the military is wargaming-in its simplest form this involves manual wargaming.
View Article and Find Full Text PDFThe status of golden loaches (genus ) in the region of Central Europe and Balkans is still ambiguous. The greatest controversy is caused by species and . Both species are characterized by a wide spectrum of morphological variability and overlapping of distinguishing features, which then lead to difficulties in their determination.
View Article and Find Full Text PDFUnlabelled: SIMToolbox is an open-source, modular set of functions for MATLAB equipped with a user-friendly graphical interface and designed for processing two-dimensional and three-dimensional data acquired by structured illumination microscopy (SIM). Both optical sectioning and super-resolution applications are supported. The software is also capable of maximum a posteriori probability image estimation (MAP-SIM), an alternative method for reconstruction of structured illumination images.
View Article and Find Full Text PDFSingle-molecule localization microscopy methods offer high spatial resolution, but they are not always suitable for live cell imaging due to limited temporal resolution. One strategy is to increase the density of photoactivated molecules present in each image, however suitable analysis algorithms for such data are still lacking. We present 3denseSTORM, a new algorithm for localization microscopy which is able to recover 2D or 3D super-resolution images from a sequence of diffraction limited images with high densities of photoactivated molecules.
View Article and Find Full Text PDFWe introduce and demonstrate a new high performance image reconstruction method for super-resolution structured illumination microscopy based on maximum a posteriori probability estimation (MAP-SIM). Imaging performance is demonstrated on a variety of fluorescent samples of different thickness, labeling density and noise levels. The method provides good suppression of out of focus light, improves spatial resolution, and allows reconstruction of both 2D and 3D images of cells even in the case of weak signals.
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