Publications by authors named "P Kasap"
Sepsis is the most lethal and expensive condition treated in intensive care units. Sepsis survivors frequently suffer long-term cognitive impairment, which has been linked to the breakdown of the blood-brain barrier (BBB) during a sepsis-associated "cytokine storm". Because animal models poorly recapitulate sepsis pathophysiology, human models are needed to understand sepsis-associated brain injury and to develop novel therapeutic strategies.
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- The microSiM (µSiM) is an innovative membrane-based culture platform designed to effectively model the blood-brain barrier (BBB), offering improved features for live cell imaging and easier assessment of cell interactions.
- It supports both monoculture and co-culture systems using ultrathin nanoporous membranes, compatible with various cell types including primary cultures and human induced pluripotent stem cells (hiPSCs).
- The platform facilitates qualitative analysis through immunofluorescence staining and allows for quantitative evaluation of barrier function, promoting advancements in tissue chip technology for studying human tissues.
Article Synopsis
- Advanced in vitro tissue chip models, like the modular µSiM (m-µSiM), can help reduce animal testing and support potential "on-chip" clinical trials.
- The m-µSiM uses easy-to-produce components to allow labs to quickly assemble and adjust designs without needing advanced microfabrication skills.
- Demonstrations of the m-µSiM's effectiveness include replicating blood-brain barrier properties, ensuring reliable results across different labs, and showcasing its flexibility for various studies by enabling quick modifications and additions.
We describe the extended endothelial cell culture method (EECM) for the differentiation of human pluripotent stem cells (hPSCs) into brain microvascular endothelial cell (BMEC)-like cells. EECM-BMEC-like cells resemble primary human BMECs in morphology, molecular junctional architecture, and diffusion barrier characteristics. A mature immune phenotype with proper endothelial adhesion molecule expression makes this model distinct from any other hPSC-derived blood-brain barrier (BBB) model and suitable to study immune cell migration across the BBB in a disease relevant and personalized fashion.
View Article and Find Full Text PDFInvestigation the anterior mandibular lingual concavity by using cone-beam computed tomography.
Folia Morphol (Warsz)
December 2021
Article Synopsis
- The study investigates the presence of lingual concavity in the mandible, which can lead to serious intraoral bleeding during surgery, particularly in the interforaminal region.
- A total of 106 patients' cone-beam computed tomography images were analyzed, revealing that 77.9% exhibited type III lingual concavity, with significant variations in concavity angle and depth.
- The findings suggest that using CBCT imaging can help identify lingual concavity, potentially preventing complications from cortical perforation in surgical settings.