Publications by authors named "P K Ponnuswamy"
Article Synopsis
- Hemophilia B is an X-linked bleeding disorder caused by mutations in the FIX gene, necessitating continuous FIX replacement therapy, often through complex intravenous methods.
- This study focused on improving new rIX-FP variants, specifically R338L and R338L/E410K, to enhance their effectiveness and explore their potential for subcutaneous administration.
- Results showed that the R338L variant had 4-5 times greater specific activity and was effective in reducing bleeding, requiring significantly lower protein amounts compared to standard treatments.
Background: There is an emerging concept that in addition to circulating coagulation factor IX (FIX), extravascular FIX contributes to hemostasis.
Objective: Our objective was to evaluate the efficacy of extravascular FIX using animal models of tail clip bleeding and ferric chloride-induced thrombosis.
Methods: Mutant rFIX proteins with described enhanced (rFIX) or reduced (rFIX) binding to extracellular matrix were generated and characterized using aPTT, one-stage clotting, and modified FX assays.
Coronaviruses are responsible for several epidemics, including the 2002 SARS, 2012 MERS, and COVID-19. The emergence of recent COVID-19 pandemic due to SARS-CoV-2 virus in December 2019 has resulted in considerable research efforts to design antiviral drugs and other therapeutics against coronaviruses. In this context, it is crucial to understand the biophysical and structural features of the major proteins that are involved in virus-host interactions.
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- Adaptive immune responses play a role in the development of atherosclerosis, with type 1 responses being harmful and type 2 responses providing protection.
- The study focused on thymic stromal lymphopoietin (TSLP), a cytokine important for type 2 immune responses, to see if it was crucial for the anti-atherogenic effects of Freund's adjuvant.
- Results showed that Freund's adjuvant induced TSLP expression through different mechanisms in male and female mice, and TSLP signaling was essential for reducing atherosclerosis, as ApoE mice had less atherogenesis compared to ApoE/TSLPR mice that lacked this signaling.
The mechanism of the efficacy of Intravenous immunoglobulins (IVIG) in autoimmune and inflammatory diseases is not well understood. This study aimed at understanding mechanisms of IVIG-mediated suppression of effector cell activities of peripheral blood mononuclear cells (PBMC) in antibody-dependent cellular cytotoxicity (ADCC). We were particularly interested in CD56 NK cells, the main ADCC effector cells in PBMC.
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