Characterization and expression of the Neurospora crassa nmt-1 gene.

The Neurospora crassa homologue of the yeast no message in thiamine ( nmt-1) gene was characterized. The deduced 342-amino-acid gene product has more than 60% identity with other fungal homologues and 42% similarity to a putative bacterial permease. In addition to three introns disrupting the coding sequence, a differentially spliced intron in the 5' untranslated region was also detected.

A gene required for nuclear migration in Neurospora crassa codes for a protein with cysteine-rich, LIM/RING-like domains.

The ro mutants of Neurospora crassa are defective in nuclear migration and hyphal morphogenesis. Several of the ro loci have recently been shown to encode components of the dynein/dynactin motor complex. Here we report on the cloning and characterization of the ro-2 gene which codes for a novel 80 kDa protein that has two Cys-rich motifs which resemble zinc-binding LIM or RING domains thought to mediate protein-protein interactions.

Cloning and characterization of a Neurospora crassa ribosomal protein gene, crps-7.

A Neurospora crassa ribosomal protein gene, crps-7, was isolated from a genomic DNA library closely linked to the morphological gene ro-2. Sequence analysis and a computerized database search revealed a high degree of homology to the Xenopus laevis rps8 and rat rps7 gene, as well as to uncharacterized ORFs from two yeast species. Comparison with a nearly full-length cDNA clone revealed two introns, one of which is in a conserved position shared with the Xenopus gene.

A fungal actin-related protein involved in nuclear migration.

The ro-4 mutant of the filamentous fungus Neurospora crassa forms distinctive colonies in which hyphae grow into rope-like aggregates. This unusual morphology coincides with a defect in hyphal nuclear migration. The ro-4 gene was cloned from a cosmid library by complementation of the closely linked pab-2 gene.

A deletion derivative of the kalilo senescence plasmid forms hairpin and duplex DNA structures in the mitochondria of Neurospora.

A novel deletion derivative, delta kal, of the kalilo senescence plasmid from Neurospora intermedia, was recovered from a culture treated with chloramphenicol. The deletion derivative exists in mitochondria as two different, equally abundant forms: a 2.8 kb duplex DNA molecule (delta kal-2.