Super-Resolved Fluorescence Lifetime Imaging of Single Cy3 Molecules and Quantum Dots Using Time-Correlated Single Photon Counting with a Four-Pixel Fiber Optic Array Camera.

Time-resolved single molecule localization microscopy (TR-SMLM) with a 2 × 2 pixel fiber optic array camera was combined with time-correlated single photon counting (TCSPC) to obtain super-resolved fluorescence lifetime images of individual Cy3 dye molecules and individual colloidal CdSe/CdS/ZnS core/shell/shell semiconductor quantum dots (QDs). The characteristic blinking and bleaching behavior of the Cy3 and the blinking behavior of the QD emitters were used as distinguishing optical characteristics to isolate them and determine their centroid locations with spatial resolution below the optical diffraction limit. TCSPC was used to characterize the fluorescence lifetime and intensity corresponding to each emitter location.

Selection of Honey Bee () Genotypes for Three Generations of Low and High Population Growth of the Mite .

Honey bee () population declines have been associated with the parasitic mite, , which is currently primarily controlled by the use of acaricides. An alternative is to breed for resistance to , which was conducted in this study by bidirectional selection for mite fall to obtain colonies with low (resistant) or high (susceptible) population growth (LVG and HVG, respectively). Selection for three generations resulted in approx.

Cell-Free Extracts of the Ginseng Soil Bacterium Promote Suppression of Resistance of American Ginseng () to Root Rot Caused by .

A prior report showed that soil previously planted with American ginseng () contained compound(s) which could reduce ginseng resistance to root infection by , and this was not found in extracts from ginseng roots or soils not previously planted with ginseng. However, the origin of this ginseng-related factor in ginseng soils is unknown. An isolate of obtained from soil where had been harvested grew more in culture media when ginseng root extract was included, indicating the use of compounds in the extract as nutrients.