Publications by authors named "P J Gardina"
Inflammation in response to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection drives severity of coronavirus disease 2019 (COVID-19) and is influenced by host genetics. To understand mechanisms of inflammation, animal models that reflect genetic diversity and clinical outcomes observed in humans are needed. We report a mouse panel comprising the genetically diverse Collaborative Cross (CC) founder strains crossed to human ACE2 transgenic mice (K18-hACE2) that confers susceptibility to SARS-CoV-2.
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- - The study focuses on Th17 cells, which are known for their role in autoimmune diseases; however, the differentiation pathways and memory structure of these cells in humans are still not fully understood.
- - Researchers utilized surface markers like CCR6 to examine human type 17 memory cells, revealing a continuum of cell types influenced by RORγt levels that reflects early activation preferences.
- - While the properties of CCR6+ cells remain stable under non-inflammatory conditions, varying activation environments can further alter their functions, showcasing both adaptability and a complex memory in type 17 cells.
Article Synopsis
- Pro-inflammatory T cells, specifically Th17 cells, express multiple chemokine receptors, but their individual functions were previously unclear, particularly focusing on CCR6 and CCR2.
- Our research identified a specific subgroup of CD4CCR6 T cells that not only have a pathogenic Th17 signature but can also produce inflammatory cytokines without TCR activation and are highly efficient in transendothelial migration (TEM).
- We found that while CCR6 can help these cells arrest on activated endothelial cells, it does not facilitate TEM, and CCR2 is critical for TEM despite not mediating arrest; this suggests that the functions of chemokine receptors in lymphocyte migration are complex and context-dependent.
Article Synopsis
- Th17 cells have mostly been studied in mice for their role in autoimmune diseases, but their differentiation and memory structures in humans remain poorly understood.
- Researchers used varying levels of surface CCR6 to show that human type 17 memory cells exist in a continuum that reflects their developmental pathways, influenced by the protein RORγt.
- The phenotypes and epigenomes of these CCR6 cells are stable, but different activation conditions can lead to new functionalities, demonstrating the unique adaptability of type 17 cells.
Inflammation in response to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection drives severity of coronavirus disease 2019 (COVID-19) and is influenced by host genetics. To understand mechanisms of inflammation, animal models that reflect genetic diversity and clinical outcomes observed in humans are needed. We report a mouse panel comprising the genetically diverse Collaborative Cross (CC) founder strains crossed to human ACE2 transgenic mice (K18-hACE2) that confers susceptibility to SARS-CoV-2.
View Article and Find Full Text PDF