Quantitative zymography of matrix metalloproteinases by measuring hydroxyproline: application to gelatinases A and B.

Gelatinases A and B are metalloproteinases involved in the degradation of the extracellular matrix. Detection and quantification of these enzymes in physiological and pathological conditions such as rheumatoid arthritis, tumor invasion and metastasis may be clinically useful. Gelatin zymography is an electrophoretic technique specific for gelatinases.

Sufentanil with and without diazepam for coronary artery bypass graft surgery.

We studied the sufentanil-sparing effect of diazepam for anesthesia induction and compared stress responses (hemodynamics, plasma catecholamine levels) and sufentanil and diazepam plasma levels during sufentanil anesthesia with or without diazepam. Sixteen aortocoronary bypass surgery patients were randomly assigned to receive diazepam (D) 100 micrograms/kg (Group DS) or not (Group S), then sufentanil (Sf) infused at 150 micrograms/min until unconsciousness (U). After tracheal intubation, the Sf dose for U (Sfu) was repeated, preceded in Group DS by D 150 micrograms/kg.

Epinephrine-aminophylline-induced arrhythmias after midazolam or thiopentone in halothane-anaesthetized dogs.

The purpose of this study was to evaluate epinephrine-aminophylline-induced arrhythmias during halothane anaesthesia after induction with thiopentone or midazolam. Ten mongrel dogs were studied during 1 MAC halothane and 50% N2O:O2 anaesthesia while maintaining constant acid-base status. The minimal arrhythmogenic infusion rate of epinephrine (MAIRE) and the corresponding plasma concentration of epinephrine (MAPC) required to produce ventricular arrhythmias before and after aminophylline were higher following induction of anaesthesia with midazolam than with thiopentone (P less than 0.

Effect of calcium entry and beta blockade during infrarenal aortic clamping.

Clamping and declamping during aortic surgery produce a hemodynamically significant myocardial stress. The cardiovascular (CV) response to this stress may be adversely altered by calcium antagonists and beta-adrenoreceptor blockade employed to control symptomatic coronary artery disease. This study evaluated the effect of verapamil (V), propranolol (P), and their combination (P + V) on the CV response to infrarenal abdominal aortic cross-clamping and declamping in anesthetized dogs.

Effects of nifedipine on the hemodynamic response to clamping and declamping of the abdominal aorta in dogs.

Clamping and declamping of the infrarenal abdominal aorta may adversely affect cardiovascular function, particularly in the presence of heart disease. This effect may be further altered by drugs used in the treatment of symptomatic coronary artery disease. The effect of nifedipine on the hemodynamic response to aortic clamping and declamping was determined in 12 dogs anesthetized with 50% nitrous oxide and 0.