Integrity of the preimplantation pig blastocyst during expansion and loss of polar trophectoderm (Rauber cells) and the morphology of the embryoblast as an indicator for developmental stage.

The embryonic ectoderm of the pig differentiated and became part of the outer barrier of the blastocyst (earlier formed by the trophectoderm alone) before shedding of the overlying polar trophectoderm around Day 10, thus securing the integrity of the rapidly expanding blastocyst. Ferritin, added to the medium of the blastocyst, was taken up rapidly by trophectoderm cells, but did not reach the blastocoele, and consequently no tracer was found within hypoblast cells. Embryonic ectoderm cells did not absorb the macromolecule, before or after loss of the polar trophectoderm.

AN EMPIRICAL COMPARISON OF THE ACCURACY OF SELECTED MULTIVARIATE CLASSIFICATION RULES.

This study involved two phases: first, when classification was based on the calibration sample; and second, in a cross-validation setting. Computer generated data were used. Results obtained from rules based on probabilities of group membership were compared for accuracy when classifying in the discriminant space and in the predictor variable spaces.