Continuous Production of Influenza VLPs Using IC-BEVS and Multi-Stage Bioreactors.

The insect cell-baculovirus expression vector system (IC-BEVS) has been an asset to produce biologics for over 30 years. With the current trend in biotechnology shifting toward process intensification and integration, developing intensified processes such as continuous production is crucial to hold this platform as a suitable alternative to others. However, the implementation of continuous production has been hindered by the lytic nature of this expression system and the process-detrimental virus passage effect.

Heterotypic spheroids as a strategy for 3D culture of cryopreserved primary human hepatocytes in stirred-tank systems.

Primary human hepatocytes (PHHs) are the preferred cell source to address liver function. Despite originating from the native tissue, one of the bottlenecks when using primary material is the donor-to-donor variability. Cryopreserved PHHs offer a high number of cells from the same donor and standardization of cell isolation and cryopreservation procedures, mitigating some of the inter-donor variability.

Bench scale Layer-by-Layer microencapasulation of Lactiplantibacillus plantarum WCFS1.

Layer-by-Layer (LbL) self-assembly encapsulation is a promising technology for the protection and delivery of lactic acid bacteria. However, laboratory-scale encapsulation is often time-consuming, involves intensive protocols tailored for small-scale operations, requires substantial amounts of energy and water, and results in a low yield of encapsulated biomass. Scaling-up this process to a bench-bioreactor scale is not simply a matter of increasing culture volume as different key parameters (not particularly relevant at lab scale) become critical, including biomass production, the number of polymer layers, and the biomass-to-polymer mass ratio.

The Influence of Diet and Obesity in Lynch Syndrome: What Do We Know So Far.

Of all new cases of colorectal cancer, Lynch syndrome (LS) accounts for approximately 3%. This syndrome is the most common hereditary cancer syndrome and is caused by pathogenic variants in the genes responsible for DNA mismatch repair. Although the relationship between colorectal cancer risk and diet is well established, little is known regarding the influence of diet and nutritional characteristics on LS's clinical evolution.

Analysis of Synovial Fluid Metabolic Profile in Patients with Knee Osteoarthritis Using Spectroscopic Magnetic Resonance Metabolomics.

 The present study aimed to evaluate the metabolic profile of synovial fluid in patients with knee osteoarthritis (KOA) and its correlation with clinical data.  We collected synovial fluid samples from the knees of 50 subjects with KOA undergoing total knee arthroplasty from October 2019 to December 2020. For each patient, we evaluated the clinical data from the medical record, the radiographic osteoarthritis grade, and the preoperative fasting blood glucose levels.