Protein microchips: use for immunoassay and enzymatic reactions.

Different proteins such as antibodies, antigens, and enzymes were immobilized within the 100 x 100 x 20-microm gel pads of protein microchips. A modified polyacrylamide gel has been developed to accommodate proteins of a size up to 400,000 daltons. Electrophoresis in the microchip reaction chamber speeded up antigen-antibody interactions within the gel.

Manual manufacturing of oligonucleotide, DNA, and protein microchips.

A simple procedure for manufacturing microchips containing various gel-immobilized compounds is described. A gel photopolymerization technique is introduced to produce micromatrices of polyacrylamide gel pads (25 x 25 x 20 microm and larger) separated by a hydrophobic glass surface. A pin device for the manual application of a compound in solution onto the activated polyacrylamide gel pad for immobilization is described.

[Chemiluminescence fiber optic immunosensor for detecting antibodies to the influenza virus].

A new variant of an optoimmunosensor for determination of antibodies to the influenza virus has been elaborated. Its advantages as compared to the traditional solid phase immunoenzyme analysis in respect to sensitivity and expressiveness are demonstrated. Time of the sensor response is below 17 min.

[A method of solid phase immunoenzyme analysis using antigen molecules immobilized on membranes].

The enzyme-linked immunoassay modification has been worked out. The method combines advantages of membrane technology of antigen immobilization which is used in the enzyme immunosensory technique and of conventional enzyme-linked immunosorbent assay. The nitrocellulose and polypropylene membranes are used as a solid-phase.

[The lactate oxidase activity of Aerococcus viridans cultures].

The oxidase activity of Aerococcus viridans is a result of the functioning of aerobic NAD-independent lactate dehydrogenase. Two fractions of flavin-containing protein which oxidize D- and L-isomers of lactic acid are revealed during the enzymic complex electrophoresis in PAAG. The enzymic complex and eluates of both fractions possess the antagonistic activity relative to 27 test-cultures of bacteria.