Publications by authors named "P I Thakore"
Glucocorticoid excess causes bone loss due to decreased bone formation and increased bone resorption; miR-433-3p is a miRNA that negatively regulates bone formation in male mice by targeting Runx2 as well as RNAs involved in Wnt, protein kinase A and endogenous glucocorticoid signaling. To examine the impact of miR-433-3p on glucocorticoid-mediated bone loss, transgenic mice expressing a miR-433-3p tough decoy inhibitor in the osteoblast lineage were administered prednisolone via slow-release pellets. Bone loss was greater in control mice treated with prednisolone compared with miR-433-3p tough decoy mice due to higher osteoclast activity in the controls.
View Article and Find Full Text PDFBackground: We investigated the relationship between neighbourhood income quintile and mental health service use by immigration experience among youth and explored changes during the COVID-19 pandemic.
Method: We used administrative data to examine mental health service use among youth aged 10 to 24 in British Columbia, Canada, between April 1, 2019, and March 31, 2022. We compared rates of community-based mental health service use, emergency department visits, and hospitalizations and the proportion of involuntary admissions by neighbourhood income quintile and immigration.
Emerging small noncoding RNAs (sncRNAs), including tRNA-derived small RNAs (tsRNAs) and rRNA-derived small RNAs (rsRNAs), are critical in various biological processes, such as neurological diseases. Traditional sncRNA-sequencing (seq) protocols often miss these sncRNAs due to their modifications, such as internal and terminal modifications, that can interfere with sequencing. We recently developed panoramic RNA display by overcoming RNA modification aborted sequencing (PANDORA-seq), a method enabling comprehensive detection of modified sncRNAs by overcoming the RNA modifications.
View Article and Find Full Text PDFArticle Synopsis
- The study investigates how the balance between T helper type 1 (T1) and other T cell types is maintained, which is crucial for effective antiviral and anti-tumor responses.
- Researchers utilized a specialized culture system and CRISPR screens to uncover regulators that influence T cell differentiation, focusing on the role of RAMP3 and its interactions with the neuropeptide CGRP.
- Findings reveal that after viral infection, neuron-produced CGRP enhances T1 and CD8 T cell responses via RAMP3, creating a neuroimmune circuit that aids in controlling acute viral infections.
Article Synopsis
- Optical pooled screening (OPS) is a method that connects images of cells with genetic changes, but it previously had limitations in its ability to analyze complex data in cancer cell lines.
- The new technology, PerturbView, improves OPS by amplifying genetic barcodes for more detailed and varied phenotype analysis across different biological systems, including stem cells and immune cells.
- PerturbView has unveiled both known and new regulatory mechanisms in immune pathways, and it can be integrated with spatial transcriptomics, enhancing the potential for comprehensive studies of cellular behaviors in complex tissue environments.