[Clinical and pathogenetic significance of collagen metabolism disorder in children with gastroesophageal reflux disease].

In 62 children with gastroesophageal reflux disease (GERD) and 32 with gastroduodenitis (DG) aged 9-17 years, the peculiarities of metabolism of collagen were studied. High levels of fractions of sialic acids were set, that was associated with the protein fructose, fractions of hydroxyproline in children with GERD compared with the patients with DG, which testify to the process of degradation of collagen and may be one of the factors contributing to the local inflammation of the esophagus and gastroduodenal zone of the digestive tract. The prevalence of Helicobacter pylori, as well as violations of diet, play an important role in maintaining the inflammatory process.

[A procedure for determination of acid mannosidase activities in biological fluids].

A procedure has been developed for simultaneous determination of the activities of alpha-D- and beta-D-mannosidase in the biological fluids from the quantity of free 4-nitrophenol. The latter is released via enzymatic degradation of substrates of 4-nitrophenyl-alpha-D-mannose and 4-nitrophenyl-beta-D-mannose in individual incubation tests.

[A technique for determination of free and peptide-bound hydroxyproline in blood serum].

A technique has been developed to determine free and peptide-bound hydroxyproline in blood serum. It is based on the principle of chloramine oxidation of hydroxyproline and condensation of oxidation products with p-dimethylaminobenzaldehyde. Data on changes in the serum level of hydroxyproline in children with acute pneumonia and in pregnant women are presented.

[Age-related changes in the exchange of hexosamine-containing biopolymers in rats under immobilization stress].

Daily double spinal immobilizations (for 60 minutes, at intervals of 7-8 hours) for 3 days were studied for their impact on the metabolism of hexosamine-containing biopolymers in the mucosa of the stomach and lung in male rats of different ages (kids, adults, and olds). At the same time, the highest increases in the level of hexosamine glycane, glycoprotein, and hexosamine-containing glycopeptides and a rise in the activity of the lysosomal enzymes N-acetyl-beta-D-hexosaminidase (EC 3.2.

[Detection of serum fucosidase activity].

A procedure has been developed for simultaneous determination of the activity of a-L-fucosidase (EC 3.2.1.