Publications by authors named "P I Pozdnyakov"
Article Synopsis
- Hypermethylation in gene regulatory regions is linked to various cancers, prompting research into its implications for lung cancer detection.
- The study utilized GLAD-PCR assay to identify specific methylated sites in 13 downregulated genes within lung cancer tissue samples.
- Findings reveal that certain RCGY sites in genes like MYF6 and TERT show promising diagnostic potential, achieving 80% sensitivity and 88% specificity for lung cancer detection compared to normal tissues.
Salmon calcitonin (sCT) was prepared in good yield and high purity by the condensation of Nalpha-Boc-cyclic decapeptide, Boc-C1SNLSTC7VLG-OH (1,7-disulfide), with protected docosapeptide (Psc)LSQE(OPse)LHK(Psc)LQTYPRTNTGSGTP-NH2 x 3TFA, followed by deprotection of Boc with trifluoroacetic acid and Psc/Pse with piperidine. The 2-(phenylsulfonyl)ethoxycarbonyl (Psc) and 2-(phenylsulfonyl)ethyl (Pse) protecting groups were recently developed. The two peptides were built up by stepwise and fragment condensation using appropriate Nalpha-Boc-amino acids and subsequent deprotection in solution.
View Article and Find Full Text PDFA new approach to induce directed mutations in genes of study through simple cotransfection of E. coli cells by the mixture of primer and template was developed. This method is based on the use of synthetic phosphotriester analogues of oligonucleotides as site-specific mutagenic primers.
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