Repair of UV-induced damage in wild-type and mutant strains of Schizophyllum commune.

The basidiomycete fungus Schizophyllum commune was found to have both photo-repair and dark-repair systems for UV-induced damage. Three UV-sensitive mutants were isolated and characterized for ability to repair UV-induced damage in light and dark, and for cross-sensitivity to caffeine and methyl methanesulfonate. Two of the mutants were damaged, to different extents, in their capacity for excision repair; one of these mutants was also probably damaged in post-replication repair.

Selection with cycloheximide of metabolic and UV-sensitive mutants of Schizophyllum commune and Saccharomyces cerevisiae.

The difference in lethality to cycloheximide between actively dividing and non-dividing cells was exploited to enhance detection of auxotrophic and UV-sensitive mutants in the fungi Schizophyllum commune and Saccharomyces cerevisiae.