Publications by authors named "P Humblot"
Endometritis is an inflammatory disease that negatively influences fertility and is common in milk-producing cows. An in vitro model for bovine endometrial inflammation was used to identify enrichment of -acting regulatory elements in differentially methylated regions (DMRs) in the genome of in vitro-cultured primary bovine endometrial epithelial cells (bEECs) before and after treatment with lipopolysaccharide (LPS) from , a key player in the development of endometritis. The enriched regulatory elements contain binding sites for transcription factors with established roles in inflammation and hypoxia including NFKB and Hif-1α.
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- Subclinical endometritis (SCE) is an inflammation affecting postpartum dairy cows' fertility, leading researchers to explore blood-based diagnostic biomarkers instead of invasive methods.
- A study compared the miRNA profiles of healthy and SCE-affected cows, revealing 34 differentially abundant miRNAs that also interact with thousands of gene transcripts linked to important biological processes.
- The analysis showed that miRNA profiles could serve as potential diagnostic tools, with significant differences observed at 21 days postpartum compared to 44 days postpartum, although no clear stage effect was identified.
Article Synopsis
- Lipopolysaccharide (LPS) from Gram-negative bacteria is linked to mastitis and metritis in dairy cattle, causing inflammation in the endometrium and stimulating proliferation of endometrial epithelial cells.
- A transcriptomic study identified 752 and 727 differentially expressed genes (DEGs) related to proliferation and apoptosis after exposing bovine endometrial epithelial cells to various concentrations of LPS.
- The analysis revealed 116 potential transcription factors and highlighted key signaling pathways, showing that LPS significantly activates pro-inflammatory and cell proliferation pathways, which may explain its role in endometrial issues.
In postpartum dairy cows, subclinical endometritis (SCE) is characterized by persistent endometrial inflammation, which has profound detrimental effects on subsequent reproductive performance. To date, transcriptomic studies related to this condition were either based on biopsy-derived whole-endometrium tissue or endometrial swab or cytobrush samples, thus masking effects of disease on cell type-specific gene expression. This study tested the hypothesis that different endometrial health statuses are associated with distinct transcription profiles of endometrial stromal, glandular, and luminal epithelial cells.
View Article and Find Full Text PDFBackground: The endometrium is a heterogeneous tissue composed of luminal epithelial (LE), glandular epithelial (GE), and stromal cells (ST), experiencing progesterone regulated dynamic changes during the estrous cycle. In the cow, this regulation at the transcriptomic level was only evaluated in the whole tissue. This study describes specific gene expression in the three types of cells isolated from endometrial biopsies following laser capture microdissection and the transcriptome changes induced by progesterone in GE and ST cells.
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