Arxula adeninivorans recombinant guanine deaminase and its application in the production of food with low purine content.

Purines of exogenous and endogenous sources are degraded to uric acid in human beings. Concentrations >6.8 mg uric acid/dl serum cause hyperuricemia and its symptoms.

Arxula adeninivorans recombinant urate oxidase and its application in the production of food with low uric acid content.

Hyperuricemia and its symptoms are becoming increasingly common worldwide. Elevated serum uric acid levels are caused by increased uric acid synthesis from food constituents and reduced renal excretion. Treatment in most cases involves reducing alcohol intake and consumption of meat and fish or treatment with pharmaceuticals.

Arxula adeninivorans recombinant adenine deaminase and its application in the production of food with low purine content.

Aims: Construction of a transgenic Arxula adeninivorans strain that produces a high concentration of adenine deaminase and investigation into the application of the enzyme in the production of food with low purine content.

Methods And Results: The A. adeninivorans AADA gene, encoding adenine deaminase, was expressed in this yeast under the control of the strong inducible nitrite reductase promoter using the Xplor(®) 2 transformation/expression platform.

Arxula adeninivorans xanthine oxidoreductase and its application in the production of food with low purine content.

Aims: Isolation and characterization of xanthine oxidoreductase and its application in the production of food with low purine content.

Methods And Results: The A. adeninivorans xanthine oxidoreductase is an inducible enzyme.

Studies of the Enzymic Capacities and Transport Properties of Pea Root Plastids.

Plastids have been isolated from pea (Pisum sativum L.) roots with a high degree of purity and intactness. In these plastids, the activity of enzymes involved in carbohydrate metabolism have been analyzed and corrected for cytosolic contamination.