Dose-dependent sensitivity of human 3D chromatin to a heart disease-linked transcription factor.

Dosage-sensitive transcription factors (TFs) underlie altered gene regulation in human developmental disorders, and cell-type specific gene regulation is linked to the reorganization of 3D chromatin during cellular differentiation. Here, we show dose-dependent regulation of chromatin organization by the congenital heart disease (CHD)- linked, lineage-restricted TF TBX5 in human cardiomyocyte differentiation. Genome organization, including compartments, topologically associated domains, and chromatin loops, are sensitive to reduced dosage in a human model of CHD, with variations in response across individual cells.

Moderating effects of demographic characteristics on the relationship between parenting practices and energy balance related behaviors of Chinese preschoolers.

Background: Early establishment of energy balance related behaviors (EBRBs) may be effective in combating unhealthy lifestyle in preschoolers. Parents are responsible for cultivating preschoolers' EBRBs directly through parenting practices. Although investigating the impact of various parenting practices on preschoolers' EBRBs is crucial to determine which practices should be recommended to parents to help reverse childhood unhealthy lifestyle, it is important to assess whether these effects of parenting practices on preschoolers' EBRBs would be similar across different groups of preschoolers, necessitating research into the moderating effects of demographic characteristics.

Single-cell analysis of the epigenome and 3D chromatin architecture in the human retina.

Most genetic risk variants linked to ocular diseases are non-protein coding and presumably contribute to disease through dysregulation of gene expression, however, deeper understanding of their mechanisms of action has been impeded by an incomplete annotation of the transcriptional regulatory elements across different retinal cell types. To address this knowledge gap, we carried out single-cell multiomics assays to investigate gene expression, chromatin accessibility, DNA methylome and 3D chromatin architecture in human retina, macula, and retinal pigment epithelium (RPE)/choroid. We identified 420,824 unique candidate regulatory elements and characterized their chromatin states in 23 sub-classes of retinal cells.

Amplicon sequences of sourdough starter cultures treated with varying levels of water chlorination.

Here, we present amplicon sequences from sourdough starter cultures that have been treated with a chlorine concentration gradient mirroring public water distribution systems. Data derived present insights into the effect of important environmental factors that may influence the formation of microbial communities in food biomes.

Weightism in Asia: A Narrative Review and Implications for Practice.

Weightism, also known as weight-related discrimination, is pervasive and believed to be one of the socially accepted types of discrimination in Asia. Weightism is pervasive, impactful, and has significant repercussions on individuals grappling with excess weight. Despite being a major risk factor for obesity, excess weight is not well documented in the Asian literature.