Synthetic transcription factors establish the function of nine amino acid transactivation domains of Komagataella phaffii Mxr1.

The zinc finger transcription factor Mxr1 (methanol expression regulator 1) of the methylotrophic yeast Komagataella phaffii (formerly Pichia pastoris) harbors a DNA-binding domain (DBD) consisting of two CH zinc fingers (Mxr1ZF) between amino acids 36-101 and a previously identified nine amino acid transactivation domain (9aaTAD) between residues 365-373 (TAD A, QELESSLNA). Beyond this, 21 putative 9aaTADs (designated TAD B-V) located between amino acids 401-1155 remain to be characterized. Here, we demonstrate that a compact synthetic transcription factor composed of Mxr1ZF and three tandem copies of TAD A can activate the transcription of Mxr1 target genes for ethanol and methanol metabolism with specificity and efficiency comparable to the full-length protein.

Effect of Two Different Penetration Depths of Micro-osteoperforation on the Rate of Orthodontic Tooth Movement: A Prospective Clinical Study.

Objective: The objective is to evaluate the effectiveness of two different penetration depths of micro-osteoperforations (MOPs) on the rate of orthodontic tooth movement over 60 days. In addition, the amount of pain and discomfort caused by the MOP was evaluated.

Materials And Methods: A total of 22 patients (18-30 years) who need fixed orthodontic treatment were recruited and randomly assigned into two groups.

Correlation of patient characteristics with peak enhancement time for pediatric cardiac computed tomography in congenital heart disease.

Objectives: Cardiac computed tomography (CCT) plays a key role in diagnosing congenital heart disease (CHD), emphasizing the need for specialized protocols in newborns due to CHD's complexity. The aim is to explore the relationship between peak enhancement time (PET) and various parameters during CHD assessment with CCT.

Material And Methods: The study involved 38 CHD patients undergoing CCT, with observations made on their heart rate, respiratory rate, saturation, cardiac output, weight, height, and age.

Iliopsoas Abscess Mimicking a Lower Motor Neuron Lesion: A Diagnostic Challenge.

Iliopsoas abscess is a rare infection that presents with a triad of fever, back pain, and hip pain. However, due to the anatomical proximity of the psoas muscle to various structures, an abscess in this region can manifest with nonspecific symptoms, leading to potential misdiagnosis and delayed diagnosis, which can be fatal. We report a case of a 54-year-old female who presented to the emergency department with right-sided flank pain and symptoms resembling lower motor neuron disorder.