S-Nitrosothiols as potential therapeutics to induce a mobilizable vascular store of nitric oxide to counteract endothelial dysfunction.

Endothelial dysfunction predisposing to cardiovascular diseases is defined as an imbalance in the production of vasodilating factors, such as nitric oxide (NO), and vasoconstrictive factors. To insure its physiological role, NO, a radical with very short half-life, requires to be stored and transported to its action site. S-nitrosothiols (RSNOs) like S-nitrosoglutathione (GSNO) represent the main form of NO storage within the vasculature.

Flush with a flash: natural three-component antimicrobial combinations based on -nitrosothiols, controlled superoxide formation and "domino" reactions leading to peroxynitrite.

-Nitrosothiols are ˙NO releasing agents renowned for vasodilatory and antioxidant properties. O˙ promotes their decomposition, forming highly aggressive peroxynitrite ions (ONOO). Since the production of O˙ can be controlled by enzymes or by visible light, such otherwise harmless components can be turned into effective antimicrobial and nematicidal combinations with numerous potential applications in medicine.

In vivo and in silico evaluation of a new nitric oxide donor, S,S'-dinitrosobucillamine.

Purpose: In a previous work, we have synthetized a new dinitrosothiol, i.e. S,S'-dinitrosobucillamine BUC(NO) combining S-nitroso-N-acetylpenicillamine (SNAP) and S-nitroso-N-acetylcysteine (NACNO) in its structure.

The onset of left ventricular diastolic dysfunction in SHR rats is not related to hypertrophy or hypertension.

Left ventricular (LV) diastolic dysfunction, particularly relaxation abnormalities, are known to be associated with the development of LV hypertrophy (LVH). Preliminary human and animal studies suggested that early LV diastolic dysfunction may be revealed independently of LVH. However, whether LV diastolic dysfunction is compromised before the onset of hypertension and LVH remains unknown.

Accurate measurement of reduced glutathione in gamma-glutamyltransferase-rich brain microvessel fractions.

Investigation of the redox status in the cerebral circulation is of great importance in order to evaluate intensity of oxidative stress-related diseases and the corresponding therapeutic effects. Changes in levels of reduced glutathione (GSH) are a major indicator of oxidative stress conditions. However, an important limitation for measurement of GSH as a biomarker is the possible presence in samples of gamma-glutamyltransferase (GGT) activity, i.