Publications by authors named "P Giuliani"
Article Synopsis
- Glioblastoma multiforme (GBM) releases extracellular vesicles (EVs) that can transfer aggressive traits to other cells, making the study of these vesicles crucial for understanding tumor behavior.
- * EV proteins are being investigated to find new biomarkers and targets for treatment, with proteomic studies primarily using "bottom-up" mass spectrometry on EVs from GBM cells and patient fluids.
- * Despite identifying many dysregulated proteins, clinical translation is challenging due to inconsistent EV isolation methods and proteome analysis, highlighting the need for standardized techniques and exploring protein mutations to improve GBM therapies.*
The production of reactive oxygen species (ROS) in the brain is homeostatically controlled and contributes to normal neural functions. Inefficiency of control mechanisms in brain aging or pathological conditions leads to ROS overproduction with oxidative neural cell damage and degeneration. Among the compounds showing therapeutic potential against neuro-dysfunctions induced by oxidative stress are the guanine-based purines (GBPs), of which the most characterized are the nucleoside guanosine (GUO) and the nucleobase guanine (GUA), which act differently.
View Article and Find Full Text PDFGuanine-based purines (GBPs) exert numerous biological effects at the central nervous system through putative membrane receptors, the existence of which is still elusive. To shed light on this question, we screened orphan and poorly characterized G protein-coupled receptors (GPRs), selecting those that showed a high purinoreceptor similarity and were expressed in glioma cells, where GBPs exerted a powerful antiproliferative effect. Of the GPRs chosen, only the silencing of GPR23, also known as lysophosphatidic acid (LPA) 4 receptor, counteracted GBP-induced growth inhibition in U87 cells.
View Article and Find Full Text PDFArticle Synopsis
- Guanosine (GUO) is a significant signaling molecule involved in various cellular processes but is still not fully understood in its neuromodulatory role.
- GUO specifically regulates potassium (K) channel activity in human neuroblastoma SH-SY5Y cells, which affects cellular excitability.
- The study findings indicate that GUO's effects are enhanced when combined with adenosine (ADO), suggesting a functional interaction between these two molecules in modulating neural activity.