Alkaloid production in Catharanthus roseus cell cultures : XII. Biosynthetic capacity of callus from original explants and regenerated shoots.

Callus derived from hypocotyls of periwinkle, Catharanthus roseus, responded to culture on nutrient media supplementedwith IAA, BA, and zeatin with shoot formation at low frequencies. However, shoot regenerating callus could be very successfully propagated and subcultured. Alkaloid profiles of callus derived from the original explants (hypocotyls) as well as callus derived from regenerated shoots were almost identical.

Cryopreservation of periwinkle, Catharanthus roseus cells cultured in vitro.

Unlabelled: A procedure for prolonged cryogenic storage of periwinkle cell cultures is described. Cells derived from periwinkle, Catharanthus roseus (L.) G.