ANCA-Associated Glomerulonephritis and Anti-Phospholipid Syndrome in a Patient with SARS-CoV-2 Infection: Just a Coincidence?

Many reports have described a high incidence of acute kidney injury (AKI) among patients with COVID-19. Acute tubular necrosis has been reported to be the most common damage in these patients, probably due to hemodynamic instability. However, other complex processes may be involved, related to the cytokine storm and the activation of innate and adaptive immunity.

Erythrocyte redox state in uremic anemia: effects of hemodialysis and relevance of glutathione metabolism.

Reduced and oxidized glutathione and pyridine coenzymes, glutathione-related enzymes and Cu,Zn-superoxide dismutase (Cu,Zn-SOD) were investigated in the RBC of patients with chronic renal failure (CRF) and in age- and sex-matched controls. The effects of hemodialysis (HD) were also studied. A defective RBC redox state was shown in the CRF group based on a decreased GSH/GSSG ratio and NADPH levels.

[Extracorporeal hemodialysis: various++ metabolic and erythrocyte-membrane features of uremic subjects].

Extracorporeal dialysis in uremic subjects produces erythrocyte alterations on energetic and redox metabolism. On this basis, we have tried to verify a fundamental parameter for the integrity of the red blood cell namely the glutathione content both in the oxidized and reduced form. Comparisons were made between two groups of subjects (similar in age, sex and number).

[Uptake of 86Rb by human erythrocytes: modification of the method and applications].

In this study we applied a method generally used for the study of Na+,K(+)-ATPase, as well as other systems of potassium transport, which makes use of a rubidium isotope (86Rb) as analogue of the potassium and is known as uptake of the 86Rb. This method proved to be particularly sensitive and versatile for kinetic studies of this pump system, allowing to assess possible alterations. Its application in the study of sodium and potassium transport in erythrocytes of uremic subjects in extracorporeal dialysis made it possible to reveal certain alterations due both to pump-dependent and pump-independent uptake.

The effect of deazaadenosine analogues on the proliferation rate of cultured Vero cells.

Five adenosine analogues resistant to adenosine deaminase were analyzed for their effects on the proliferation on cultured Vero cells. Cells were exposed for 48 hr to various concentrations of 1-deazaadenosine (c1Ado), 3-deazaadenosine (c3Ado), 7-deazaadenosine (c7Ado), 1,3-dideazaadenosine (c1,3Ado) or 1,7-dideazaadenosine (c1,7Ado) and then counted with a hemocytometer. Results indicate that 1) whereas c7Ado produced a marked decrease in cell growth, c3Ado was toxic only at the highest concentration tested (30 microM), 2) c1Ado and c1,7Ado did not affect cell replication and 3) c1,3Ado produced a significant stimulation of cell proliferation.