Publications by authors named "P G Holhorea"

Article Synopsis
  • A confinement stress test was conducted to analyze the stress response in gilthead sea bream compared to European sea bass, focusing on behavioral changes using three-axis accelerometers.
  • The study involved rearing gilthead sea bream at different stocking densities and monitoring various health metrics, showing that fish in high-density (HD) conditions had lower feed intake and growth rates, along with detectable muscle changes and minor injuries.
  • After undergoing stress tests, fish in high-density settings demonstrated quicker recovery in activity and respiration, suggesting they became accustomed to the high stocking environment over time.
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Fish were kept for six weeks at three different initial stocking densities and water O concentrations (low-LD, 8.5 kg/m and 95-70% O saturation; medium-MD, 17 kg/m and 55-75% O saturation; high-HD, 25 kg/m and 60-45% O saturation), with water temperature increasing from 19 °C to 26-27 °C. The improvement in growth performance with the decrease in stocking density was related to changes in skin and intestinal mucosal microbiomes.

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We aimed to evaluate the efficiency of hard-gelatin and hard-hydroxypropyl methylcellulose (HPMC) capsules as biodegradable alternative containers to plastic straws in European eel (Anguilla anguilla), gilthead seabream (Sparus aurata) and European sea bass (Dicentrarchus labrax) sperm cryopreservation. Sperm samples from each European eel (n = 12) were diluted 1:8:1 (sperm: extender P1+5 % egg yolk: methanol). Gilthead seabream (n = 12) samples were individually diluted in a cryoprotectant solution of 5 % MeSO + NaCl 1 % plus BSA (10 mg mL) at a ratio of 1:6 (sperm: cryoprotectant solution).

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The study combined the use of biometric, behavioral, physiological and external tissue damage scoring systems to better understand how high stocking densities drive schooling behavior and other adaptive features during the finishing growing phase of farmed gilthead sea bream in the Western Mediterranean. Fish were grown at three different final stocking densities (LD, 8.5 kg/m; MD, 17 kg/m; HD, 25 kg/m).

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This work studied the potential of a combination of pungent spices (capsicum, black pepper, ginger, and cinnamaldehyde) to be used as a supplement in diets of gilthead seabream (; 44.1 ± 4.2 g).

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