Identification of four genes expressed by feeding female Ixodes scapularis, including three with sequence similarity to previously recognized genes.

We created a cDNA library from feeding, female Ixodes scapularis ticks and screened the library with a subtracted probe to eliminate most genes common to feeding female and mating male I. scapularis ticks. Four unique genes were identified in this screen.

Mating, male Ixodes scapularis express several genes including those with sequence similarity to immunoglobulin-binding proteins and metalloproteases.

We created a cDNA library from mating, male Ixodes scapularis ticks and screened the library with a subtracted probe to eliminate genes common to feeding female and mating male I. scapularis ticks. A total of seven unique cDNAs were identified in this screen.

The prevalence of Borrelia burgdorfieri (Spirochaetales: spirochaetaceae) and the agent of human granulocytic ehrlichiosis (Rickettsiaceae: Ehrlichieae) in Ixodes scapularis (Acari:Ixodidae) collected during 1998 and 1999 from Minnesota.

We tested 103 adult Ixodes scapularis Say from 12 counties in Minnesota for the presence of Borrelia burgdorferi and the causative agent of human granulocytic ehrlichiosis (HGE), using polymerase chain reaction (PCR). A total of 17 ticks (16.5%) was positive for B.

New records of the blacklegged tick, Ixodes scapularis (Acari: Ixodidae) in Minnesota.

The Minnesota distribution of the blacklegged tick, Ixodes scapularis, was studied during 1998 and 1999. The majority of tick collecting was done by grouse hunters, who sent in specimens collected during the fall of 1998 and 1999. I.

A chemoattractant cytokine associated with granulomas in tuberculosis and silicosis.

Chronic inflammation and granuloma formation are associated with mononuclear cell infiltrates and are characteristic pathologic responses in tuberculosis. To identify host cell genes involved in tuberculous pathology, we screened macrophage cDNA libraries for genes induced by mycobacterial infection. One gene isolated in this screen, osteopontin (also known as early T lymphocyte activation protein 1 or Eta-1), was of particular interest because it is a cytokine and macrophage chemoattractant.