Separation of Protein Crystals from Spores of Bacillus thuringiensis by Ludox Gradient Centrifugation.

A method is described for the purification of Bacillus thuringiensis protein crystals by Ludox gradient centrifugation. This method is simple, inexpensive, fast, and efficient compared with other techniques. It has been successfully used to purify and characterize the protein crystals from several B.

The Kinetics of Chlorate Uptake by XD Tobacco Cells.

The uptake of [(36)Cl]chlorate by the 14U variant of the XD cell line of Nicotiana tobaccum L. cv Xanthi was investigated to examine the use of chlorate as a nitrate analog in transport studies. The kinetics of chlorate uptake against concentration was complex.

N-caffeoyl-4-amino-n-butyric acid, a new flower-specific metabolite in cultured tobacco cells and tobacco plants.

PUT cells were selected from the XD line of cultured tobacco cells (Nicotiana tabacum L. cv. Xanthi-nc) for the ability to utilize putrescine as sole nitrogen source.

Tubulin from cultured tobacco cells: isolation and identification based on similarities to brain tubulin.

The microtubule protein, tubulin, was isolated from most other proteins of cell suspension cultures of Nicotiana tabacum L. by its copolymerization with cow-brain tubulin. Cow-brain tubulin was added to the soluble protein fraction of extract from (35)S-labeled tobacco cells and subjected to two cycles of temperature-dependent assembly-disassembly (copolymerization).

Developmental changes in the potential for h(2)s emission in cucurbit plants.

Based on results obtained with leaf discs exposed to sulfate, leaves on cucurbit plants (Cucurbita pepo L. cv Small Sugar Pumpkin and Cucumis sativus cv Chipper) 1 to 2.5 weeks old have a low potential for H(2)S emission (less than 10 picomoles per min per cm(2) leaf area) in response to sulfate, whereas discs from most of the leaves on plants 3 to 4 weeks old emit H(2)S at a higher rate (50 to 150 picomoles per min per cm(2) leaf area).