Publications by authors named "P Fanjul-Bolado"
Article Synopsis
- The introduction of biological drugs for treating cancers and autoimmune diseases has changed the medical landscape, but anti-drug antibodies (ADAs) can reduce their effectiveness.
- Researchers developed an ambipolar electrolyte-gated transistor (EGT) immunosensor using reduced graphene oxide to detect low concentrations of ADAs specifically against Infliximab (IFX).
- This new sensor is easy to make, operates at low voltages, delivers quick results, and can accurately measure ADAs even when competing with natural antagonists like TNF-α.
A new spectroelectrochemical two-enzyme sensor system has been developed for the detection of acetaldehyde in wine. A combination of spectroscopy and electrochemistry improves the analytical features of the electrochemical sensor because the optical information collected with this system is only associated with acetaldehyde and avoids the interferents also present in wines as polyphenols. Spectroelectrochemical detection is achieved by the analysis of the optical properties of the K[Fe(CN)]/K[Fe(CN)] redox couple involved in the enzymatic process: aldehyde dehydrogenase catalyzes the aldehyde oxidation using β-nicotinamide adenine dinucleotide hydrate (NAD) as a cofactor and, simultaneously, diaphorase reoxidizes the NADH formed in the first enzymatic process due to the presence of K[Fe(CN)].
View Article and Find Full Text PDFLatest solutions in biotechnologies and biosensing targeted cold-active extremozymes. Analysis of acetaldehyde as a relevant quality indicator of wine is one example of application that could benefit from using low-temperatures operating catalysts. In search of novel aldehyde dehydrogenases (ALDH) with high stability and activity at low temperatures, the recombinant S2-ALDH from the Antarctic Flavobacterium PL002 was obtained by cloning and expression in Escherichia coli BL21(DE3).
View Article and Find Full Text PDFHealth monitoring is experiencing a radical shift from clinic-based to point-of-care and wearable technologies, and a variety of nanomaterials and transducers have been employed for this purpose. 2D materials (2DMs) hold enormous potential for novel electronics, yet they struggle to meet the requirements of wearable technologies. Here, aiming to foster the development of 2DM-based wearable technologies, reduced graphene oxide (rGO)-based liquid-gated transistors (LGTs) for cation sensing in artificial sweat endowed with distinguished performance and great potential for scalable manufacturing is reported.
View Article and Find Full Text PDFArticle Synopsis
- The paper discusses the design and fabrication of three innovative electrochemical measurement cells using screen-printed electrode chips (SPECs), highlighting their specific applications in flow injection, thin-layer, and batch techniques.
- Each cell has unique features, including an acrylic base and cover, ensuring easy electrical connections, containment of the SPEC to prevent damage, compatibility with various SPECs, and excellent sealing, with the flow cell allowing for customization and visual inspection.
- The thin-layer cell enables near-ideal voltammetry, while the versatile universal batch cell caters to a wide range of sample volumes and conditions, also providing novel methods for advanced electrochemical analysis.