Publications by authors named "P England"
Replication Protein A (RPA) plays a pivotal role in DNA replication by coating and protecting exposed single-stranded DNA, and acting as a molecular hub that recruits additional replication factors. We demonstrate that archaeal RPA hosts a winged-helix domain (WH) that interacts with two key actors of the replisome: the DNA primase (PriSL) and the replicative DNA polymerase (PolD). Using an integrative structural biology approach, combining nuclear magnetic resonance, X-ray crystallography and cryo-electron microscopy, we unveil how RPA interacts with PriSL and PolD through two distinct surfaces of the WH domain: an evolutionarily conserved interface and a novel binding site.
View Article and Find Full Text PDFDisparities in Evaluation, Treatment, and Outcomes of Pediatric Knee Injuries.
Sports Med Arthrosc Rev
December 2024
Similar to other fields, there is growing evidence of disparities in pediatric sports medicine. For children and adolescents with knee injuries, disparities persist along the lines of sex, gender, race, ethnicity, insurance, socioeconomic status, and other determinants. These factors influence the evaluation, treatment, and outcomes for a variety of knee pathologies, including anterior cruciate ligament ruptures, meniscus tears, osteochondritis dissecans, cartilage injuries, and tibial spine fractures.
View Article and Find Full Text PDFBackground: Patients who first meet clinical criteria for sepsis while boarding in the emergency department (ED) may not receive optimal sepsis care.
Objective: Assess the association between ED boarding status and sepsis quality of care and outcomes.
Methods: We conducted a retrospective cohort study of adult patients admitted to a large academic hospital from July 2021 to October 2023 who had clinical features consistent with sepsis present while physically in the ED.
Article Synopsis
- * Sugammadex can reverse the effects of rocuronium-induced neuromuscular blockade but may also provoke anaphylaxis, indicating a need for better treatment options.
- * Research into the antibodies of patients with rocuronium sensitivity found a diverse range that can activate anaphylaxis; high-affinity antibodies capable of reversing rocuronium effects were isolated, suggesting new avenues for diagnosis and treatment.
Article Synopsis
- - High seroreactivities to Ebola (EBOV) and Sudan (SUDV) orthoebolavirus antigens were found in rural populations of Cameroon, particularly among Pygmies, despite no reported outbreaks.
- - The study did not find a direct link between the risk of NHP (nonhuman primates) bites and seroreactivity, but indicated that exposure may vary among different ethnic groups.
- - These findings suggest a possible silent circulation of orthoebolaviruses or other unknown filoviruses in forested regions, emphasizing the need for increased surveillance and research in these areas.