Rapid identification of specific genes in E. coli by hybridization to membranes containing the ordered set of phage clones.

A simplified protocol for the hybridization of labeled oligonucleotides and other radiolabeled DNAs to membranes is described and used for the detection of specific recombinant phage in the ordered miniset collection representing virtually the entire E. coli genome.

Interrelationships and metabolic effects of fatty acids in the perfused rat liver at hyperthermic temperatures.

Livers of fasted rats were perfused for 70 min at 37 degrees-43 degrees C in the presence or absence of acetate, octanoate or palmitate. Hepatic biosynthetic capacity was assessed by measuring rates of gluconeogenesis, ureogenesis, ketogenesis and O2 consumption. In the presence of each fatty acid, gluconeogenesis, ureogenesis and oxygen consumption were maintained at 37 degrees and 42 degrees C.

Canine liver isolation-perfusion at normo- and hyperthermic temperatures with perfluorochemical emulsion (Fluosol-43).

A perfluorocarbon emulsion, Fluosol-43, was used as a blood substitute for oxygen transport during isolation-perfusion of the dog liver at 37 and 43 degrees C. Preservation of hepatic functional integrity was assessed through analysis of perfusate constituents and animal survival after perfusion. Flow to the liver during perfusion was greater than 1 ml/min/g with one-third of total flow provided through the hepatic artery and two-thirds through the protal vein.

Genetic and enzymatic characterization of the inducible glycerol dissimilatory system of Neurospora crassa.

The glycerol dissimilatory system in Neurospora crassa was analyzed through the characterization of 18 Glp- mutants which were isolated after inositol-less death and filtration enrichment. All mutants obtained by this procedure could be assigned to one of three complementation groups. The subsequent genetic characterization of these glp mutations revealed lesions on the I, II, and VI chromosomes at the glp-1, glp-2, and glp-4 loci, each of which was subjected to fine-structure analysis.

Isolation and characterization of glycerol-3-phosphate dehydrogenase-defective mutants of Neurospora crassa.

Three glycerol-nonutilizing mutants deficient in the mitochondrial glycerol-3-phosphate (G3P) dehydrogenase (EC 1.1.99.