Horse kidney neutral alpha-D-glucosidase: purification of the detergent-solubilized enzyme; comparison with the proteinase-solubilized forms.

Neutral alpha-D-glucosidase (alpha-D-glucoside glucohydrolase, EC 3.2.1.

Neutral maltase: the first human B-cell enzymatic marker reflecting terminal differentiation of mature B cells into plasma cells.

Neutral maltase activity (alpha-D-glucoside glucohydrolase; EC: 3.2.1.

Distribution of neutral maltase activity in mononuclear cells from different human lymphoid organs.

Neutral maltase activity has been determined in purified monocytes, T and B lymphocytes from human peripheral blood and major lymphoid organs. In peripheral blood as in all lymphoid organs studied, neutral maltase activity was found to be significantly higher in monocytes than in B lymphocytes. Neutral maltase activity was never detected in T lymphocytes whatever the methods used for their purification.

A single-step centrifugation method for separation of granulocytes and mononuclear cells from blood using discontinuous density gradient of Percoll.

A rapid and reproducible method is described for the simultaneous purification of mononuclear and polymorphonuclear cells, based on the use of a discontinuous gradient of Percoll. Centrifugation at 600 X gav for 20 min resulted in separation of the mononuclear and polymorphonuclear cells into 2 distinct bands at the interfaces. The upper layer contained the mononuclear cells and the lower band highly purified polymorphonuclear cells.