Survival of Prevotella intermedia (Bacteroides intermedius) in transport media.

Five transport media were selected for testing, in vitro, the survival of a pure strain of Prevotella intermedia (Bacteroides intermedius) for 6, 24, and 72 hours. Two were non-nutrient transport media (RTF and VMG IV). The three others (TG, PY, PYG) were nutrient media.

In vitro survival of Bacteroides intermedius in five transport media, alone or in the presence of Streptococcus sanguis.

The survival rate of Bacteroides intermedius was first tested in monoculture, and Streptococcus sanguis was then added in 5 different transport media; 2 nonnutritious media, the viability-preserving medium of the University of Göteborg No. IV (VMG IV), reduced transport fluid (RTF), and 3 nutritious media, thioglycolate medium (TG), peptone yeast extract medium (PY) and PY medium with 1% glucose (PYG). All manipulations were carried out in an anaerobic chamber.