Rapid determination of the rate constant of agonist-induced association of single platelets.

We have developed a rapid and convenient method for determining the rate constant of association of single platelets. Using the concentration of single platelets prior to activation and that 0.25 min (15 s) after activation, this rate constant can be determined accurately without concern for reversibility of the aggregation process or need for statistical analysis.

Interpretation of microscopic image analysis of cell nuclei.

This study shows that microscopic image analyses of nuclear DNA have common characteristics among fixation methods and tissue types. We find that microscopic imaging measurements require both nuclear area and DNA concentration to properly convey diagnostic information. Algorithms are developed which enable infiltrating lymphocytes to act as internal DNA controls for each sample.

A compartmental model of platelet aggregation in vitro: the kinetics of single platelets.

A compartmental model of platelet aggregation which accurately describes both reversible and irreversible aggregation in vitro is presented. This model is substantiated by correlative analyses of agonist-induced aggregation as monitored by both routine aggregometry and resistive counting of single platelets. Previously unresolved differences in the reported reaction order of the aggregation process are explained.