Cell- and tissue-specific glycosylation pathways informed by single-cell transcriptomics.

While single-cell studies have made significant impacts in various subfields of biology, they lag in the Glycosciences. To address this gap, we analyzed single-cell glycogene expressions in the Tabula Sapiens dataset of human tissues and cell types using a recent glycosylation-specific gene ontology (GlycoEnzOnto). At the median sequencing (count) depth, ∼40-50 out of 400 glycogenes were detected in individual cells.

Cell and tissue-specific glycosylation pathways informed by single-cell transcriptomics.

While single cell studies have made significant impacts in various subfields of biology, they lag in the Glycosciences. To address this gap, we analyzed single-cell glycogene expressions in the Tabula Sapiens dataset of human tissues and cell types using a recent glycosylation-specific gene ontology (GlycoEnzOnto). At the median sequencing (count) depth, ~40-50 out of 400 glycogenes were detected in individual cells.

DeltaNeTS+: elucidating the mechanism of drugs and diseases using gene expression and transcriptional regulatory networks.

Background: Knowledge on the molecular targets of diseases and drugs is crucial for elucidating disease pathogenesis and mechanism of action of drugs, and for driving drug discovery and treatment formulation. In this regard, high-throughput gene transcriptional profiling has become a leading technology, generating whole-genome data on the transcriptional alterations caused by diseases or drug compounds. However, identifying direct gene targets, especially in the background of indirect (downstream) effects, based on differential gene expressions is difficult due to the complexity of gene regulatory network governing the gene transcriptional processes.

Computational investigation of actuation mechanisms of droplets on porous air-permeable substrates.

We study the actuation of droplets on porous substrates by air that permeates through pores. Air pockets are created between the droplets and the substrate which, eventually, incite the droplets to a quasi-moving state. We observe this mechanism computationally and verify it experimentally, using various case studies involving water droplets of different volume that are initially pinned on a porous substrate which has been set to different inclination levels and start to slide down when actuated by permeating air.