Microencapsulating role of whey protein isolate and sucrose in protecting the cell membrane and enhancing survival of probiotic lactobacilli strains during spray drying, storage, and simulated gastrointestinal passage.

Limosilactobacillus reuteriKUB-AC5 andLactobacillus johnsoniiKUNN19-2 were spray-dried using whey protein isolate, sucrose, and whey protein isolate combined with sucrose at 130 °C and 150 °C. Differences in membrane fatty acid compositions of probiotics led to differences in cell membrane fluidity, cell injury, and cell survival during spray drying. Combination of whey protein isolate and sucrose improved survival of L.

Response surface optimization of microfluidic formulations of nanobilosomes for enhancement of aqueous solubility, digestive stability, and cellular antioxidant activity of mangiferin.

Mangiferin-loaded nanobilosomes (MGF-NBSs) were developed using microfluidic-based techniques to improve aqueous solubility, digestive stability, and cellular antioxidant activity (CAA) of mangiferin. Preliminary experiments showed that optimal formation conditions were 5:1 aqueous (water) to solvent (ethanol) phase ratio and 85 mL/min total flow rate. Further optimization using response surface methodology provided the optimal formulation (200 mg encapsulant consisting of 90.

Cosmetic potential of lignin extracts from alkaline-treated sugarcane bagasse: Optimization of extraction conditions using response surface methodology.

Each year, sugarcane bagasse, a low-priced by-product of the sugar industry, is generated in large quantities. The aim of this study was to optimize the alkaline hydrolysis condition for the extraction of lignin from sugarcane bagasse using response surface methodology combined with Box-Behnken design, and to evaluate functional properties of lignin extracts for cosmetic applications. Three process parameters were varied (NaOH solution concentrations (3-7% w/v), temperatures (115-135 °C), and times (30-60 min)).

Effects of beeswax-carboxymethyl cellulose composite coating on shelf-life stability and intestinal delivery of holy basil essential oil-loaded gelatin microcapsules.

Despite growing interests in the use of essential oils in food and feed additives to promote health benefits, several studies have indicated that microencapsulation of these sensitive materials is necessary to increase their stability and improve their release profiles. This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation. After 3-month storage, the highest surface oil content was observed in HBEO-G, followed by the 3, 1, and 2% beeswax-CMC coated microcapsules.

Combined effects of holy basil essential oil and inlet temperature on lipid peroxidation and survival of Lactobacillus reuteri KUB-AC5 during spray drying.

Lipid peroxidation induced by oxidative stress plays an important role in reducing probiotic survival during spray drying. In this study, the probiotic Lactobacillus reuteri KUB-AC5 was co-encapsulated with holy basil essential oil, HBEO (0, 6, and 12mg/ml), by spray drying using skim milk at inlet air temperatures of 130, 140, and 150°C. The microcapsule properties in terms of morphology, size, moisture content, water activity, surface oil, microencapsulation efficiency, antioxidant activity, lipid peroxidation, and cell survival were determined.