Publications by authors named "P Cassonnet"

Article Synopsis
  • Understanding how viruses like influenza A interact with cellular processes can help develop treatments targeting host cells.
  • Researchers found that specific components of the Cullin 4-RING E3 ubiquitin ligases (CRL4), essential for virus replication, change their interactions during infection.
  • The study revealed that IAV infection alters protein associations, impacting cellular functions such as protein folding and stress responses, which could lead to new antiviral strategies.
View Article and Find Full Text PDF
Article Synopsis
  • - Syncytins are genes derived from retroviruses that are crucial for the development of a syncytial structure in the placenta, influencing both placentogenesis and embryonic growth.
  • - In mice, there are two syncytiotrophoblast layers in the placenta, ST-I and ST-II, each expressing different syncytins: SynA and SynB, which interact with distinct cellular receptors for fusion.
  • - The study identified PiT1 as the receptor for SynB, and its absence leads to defects in the development of the ST-II layer, highlighting the importance of PiT1 in proper placental function.
View Article and Find Full Text PDF

Protein-protein interactions (PPIs) offer great opportunities to expand the druggable proteome and therapeutically tackle various diseases, but remain challenging targets for drug discovery. Here, we provide a comprehensive pipeline that combines experimental and computational tools to identify and validate PPI targets and perform early-stage drug discovery. We have developed a machine learning approach that prioritizes interactions by analyzing quantitative data from binary PPI assays or AlphaFold-Multimer predictions.

View Article and Find Full Text PDF

Protein-protein interactions (PPIs) offer great opportunities to expand the druggable proteome and therapeutically tackle various diseases, but remain challenging targets for drug discovery. Here, we provide a comprehensive pipeline that combines experimental and computational tools to identify and validate PPI targets and perform early-stage drug discovery. We have developed a machine learning approach that prioritizes interactions by analyzing quantitative data from binary PPI assays and AlphaFold-Multimer predictions.

View Article and Find Full Text PDF

Dimerization of SRC kinase adaptor phosphoprotein 2 (SKAP2) induces an increase of binding for most SRC kinases suggesting a fine-tuning with transphosphorylation for kinase activation. This work addresses the molecular basis of SKAP2-mediated SRC kinase regulation through the lens of their interaction capacities. By combining a luciferase complementation assay and extensive site-directed mutagenesis, we demonstrated that SKAP2 interacts with SRC kinases through a modular organization depending both on their phosphorylation-dependent activation and subcellular localization.

View Article and Find Full Text PDF