Efficacy of spectinomycin against Mycoplasma bovis induced pneumonia in conventionally reared calves.

Sixteen 3-week-old calves were intratracheally inoculated with Mycoplasma bovis. Follow-up consisted of regular bronchoalveolar lavages (BALs) and clinical examinations. Animals were slaughtered from 4 to 21 days after inoculation.

An immunohistochemical study of bovine palatine and pharyngeal tonsils at 21, 60 and 300 days of age.

An immunohistochemical study was performed on three groups of young cattle (21, 60 and 300 days of age). Tonsils (palatine and pharyngeal) and mucosae (nasal and oral) were removed. Eight monoclonal antibodies (specific for CD3, CD2, CD4, CD8, WC1, cell-surface IgM, cell-surface IgG and MHC class II molecules) and an avidin/biotin complex method on frozen sections were used.

Melatonin and prolactin secretion profile in naturally occurring scrapie in ewe.

The 24 hr pattern of melatonin secretion was determined in scrapie-affected ewes during the clinical course of the disease. The melatonin response to a night interruption by a 1 hr period of illumination was also measured. Fourteen ewes (seven control and seven scrapie-affected ewes) were subjected to artificial short days (9L:15D).

Use of monoclonal antibodies for immunohistochemical study of bovine spleen on frozen sections.

Many monoclonal antibodies reactive with bovine leukocyte differentiation antigens are now available. Immunohistochemical staining on frozen sections using these monoclonal antibodies permits study of the functional morphology of bovine spleen. This study confirms accepted notions (B and T dependent-zones) and supplies complementary data about the repartition of CD4 and CD8 cells, gamma delta T cells, MHC (Major Histocompatibility Complex) II expression, and macrophages.