A [FeFe] Hydrogenase-Rubrerythrin Chimeric Enzyme Functions to Couple H Oxidation to Reduction of HO in the Foodborne Pathogen .

[FeFe] hydrogenases are a diverse class of H-activating enzymes with a wide range of utilities in nature. As H is a promising renewable energy carrier, exploration of the increasingly realized functional diversity of [FeFe] hydrogenases is instrumental for understanding how these remarkable enzymes can benefit society and inspire new technologies. In this work, we uncover the properties of a highly unusual natural chimera composed of a [FeFe] hydrogenase and rubrerythrin as a single polypeptide.

Is there an association between 30-day mortality from out-of-hospital cardiac arrest (OHCA) and deprivation levels within Hampshire? A retrospective cohort study.

Introduction: People who live in population-dense areas, work in routine occupations, originate from a non-white background, have lower education attainment and experience a greater level of deprivation have an increased risk of suffering an OHCA and are less likely to receive bystander CPR. This study seeks to understand if these observed inequalities result in reduced survival by examining the relationship between deprivation and survival at 30 days at a UK single county level.

Methods: 30-day survival from non-traumatic OHCA in adults over 18 years of age in Hampshire from local ambulance service data (Jan 2019 - March 2023) was combined with indices of multiple deprivation (IMD) based on the home postcode.

The C2 domain augments Ras GTPase-activating protein catalytic activity.

Regulation of Ras GTPases by GTPase-activating proteins (GAPs) is essential for their normal signaling. Nine of the ten GAPs for Ras contain a C2 domain immediately proximal to their canonical GAP domain, and in RasGAP (p120GAP, p120RasGAP; ) mutation of this domain is associated with vascular malformations in humans. Here, we show that the C2 domain of RasGAP is required for full catalytic activity toward Ras.

Cold-Spray Deposition of Antibacterial Molybdenum Coatings on Poly(dimethylsiloxane).

Despite their widespread utilization in biomedical applications, these synthetic materials can be susceptible to microbial contamination, potentially compromising their functionality and increasing the risk of infection in patients. In this study, molybdenum (Mo), an essential metal in biological systems, was investigated as a Mo-based cold-sprayed coating on poly(dimethylsiloxane) (PDMS) for its potential use as biocompatible and antimicrobial surfaces for biomedical applications. Various cold-spray parameters were employed in the fabrication of Mo-embedded PDMS surfaces to alter the surface structure of the substrate, Mo loading density, and embedding layer thickness.